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KMID : 0357220090210030087
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Journal of Korean Society Physical Therapy
2009 Volume.21 No. 3 p.87 ~ p.93
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Effect of Repetitive Magnetic Stimulation on Proliferation and Viability of Adipose Tissue-Derived Stromal Cells
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Kim Su-Jeong
Park Hae-Woon
Cho Yun-Woo
Lee Joon-Ha
Seo Jeong-Min
Shin Hyun-Jin
Kang Jae-Hoon
Ahn Sang-Ho
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Abstract
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Purpose: TThis study examined the effect of repetitive magnetic stimulation (RMS) on the viability and proliferative response of human adipose tissue-derived stromal cells (hATSCs) in vitro.
Methods: The hATSCs were cultured primarily from human adipose tissue harvested by liposuction and incubated in a plastic chamber. The cells were exposed to a repetitive magnetic field using a customized magnetic stimulator (Biocon-5000, Mcube Technology). The RMS parameters were set as follows: repetition rate=10Hz, 25Hz (stimulus intensity 100%= 0.1 Tesla, at 4cm from the coil), stimulated time= 1, 5, and 20 minutes. Twenty four hours after one application of RMS, the hATSCs were compared with the sham stimulation, which were kept under the same conditions without the application of RMS. The cells were observed by optical microscopy to determine the morphology and assessed by trypan blue staining for cell proliferation. The apoptosis and viability of the hATSCs were also analyzed by fluorescence-activated cell sorting (FACS) analysis of Annexin V and MTT assay.
Results: After RMS, the morphology of the hATSCs was not changed and the apoptosis of hATSCs were not increased compared to the sham stimulation. The viability of the cells was similar to the cells given the sham stimulation. Interestingly, the level of hATSC proliferation was significantly higher in all RMS groups.
Conclusion: The application of RMS may not cause a change in morphology and viability of hATSCs but can increase the level of cell proliferation in vitro. RMS might be useful as an adjuvant tool in combination with stem cell therapy without adverse effects.
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KEYWORD
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Repetitive magnetic stimulation, Human adipose tissue-derived stromal cells, Viability, Apoptosis, Proliferation
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